Tools of Recombinant DNA Technology
Tools of Recombinant DNA Technology: Overview
This Topic covers sub-topics such as Gel Electrophoresis, Restriction Enzymes, Cloning Vectors, Electroporation, Microinjection, Plasmid as Vector, Palindromic Nucleotide Sequence, Selectable Marker, Gene Gun and, Tools of Recombinant DNA Technology
Important Questions on Tools of Recombinant DNA Technology
A recombinant DNA is formed when sticky ends of vector DNA and foreign DNA join. The sticky ends are formed by which of the following enzymes:
Agrobacterium mediated genetic transformation described as natured genetic engineering is used to resist plants from:
Which of the following technique is used for separating the fragments of DNA cut by restriction endonucleases?
Biotechnologists refer to which of the following bacteria as a natural genetic engineer of plants.
Which of the following feature of a vector is required to identify the transformed cell?
The genes (a) ori, (b) ampR and (c) rop are associated with which of the following vectors:
Fragments of DNA segments can be separated by:
In genetic engineering, which of the following is used?
The chemical method of gene transfer is used in organisms like bacteria.
Explain one chemical method of gene transfer
List any three types of chemicals used for the gene transfer method.
Use of PEG is the chemical method of gene transfer.
Heat stock is the chemical method used in molecular biology to insert DNA into the bacteria.
Which of the following methods allows the entry of recombinant DNA into the cell?
_____ is the process of increasing the permeability of the cell membrane by the application of an electric field.
What is electroporation?
_____ is a technique that uses electric energy to allow the entry of rDNA into the host cell.
What is the role of antibiotic resistance genes in genetic engineering?
Who isolated the antibiotic resistance gene?
Which are the two antibiotic resistance genes present in pBR322?
